Ensubstrates Rainbow & Ensubstrates Green’n’BlueSers-site

EnSpectr R&D department has made a true technological leap in SERS substrate development. Below we highlight some of its the most interesting features.

1. Fits any excitation wavelength

EnSpectr developed two types of SERS substrates covering the spectral range from blue to red. Universal substrates cover a wide range from blue to red and even near infra-red region. Order either “blue-green” or “rainbow” ones according to your laser wavelength

2. Seven orders EF (enhancement factor)Sers.new_

Magnetron sputtering provides both a great enhancement efficiency of the films and absolute homogeneity of EF in a single batch

3. SERS is no more just qualitative technique

Enspectr developed unique “paired” chips that allow the signal normalization to local EF variation. Available on request!

4. Price

Best price on market.


      • medical diagnostics/biotechnology
      • biological liquids components analysis (blood, urine, saliva)
      • impurities and biologically active substances ID (viruses, bacterias, toxins) in water or air
      • biomarkers
      • cytology, microbiology, biochemistry and other biomedical studies
      • DNA analysis
      • art and restoration: dyes ID on painting and other art objects
      • anti conterfeiting by marked products detection


   Ensubstrates Rainbow* Ensubstrates Blue&green*

Laser wavelength


Enhancement factor

488 nm 2.3 * 106 1.4 * 106
532 nm 107 2.2 * 106
568 nm 1.3 * 107 106
647 nm 2 * 107
780 nm 2 * 107


Substrate material glass silicon
Dimensions 20×4 mm 20×4 mm
Active areas 2xØ 4 mm 2xØ 4 mm


1 chip in a tube;

5 tubes in a vacuum packing

1 chip in a tube;

5 tubes in a vacuum packing

Storage stability Two months from development;

when unpacked – 70 hours

More than two months from development;

when unpacked – 70 hours

Recommended laser power density*** 0,1 – 0.3 mW/um2 0,5 – 1.5 mW/um2


both substrate types allow (on request) passivation with a thin transparent dielectrical layer. Useful

1) as a biosensor for sorption;

2) when treatment with ligands, antibodies, etc., is required with further acceptor deposition;

3) when substrate surface chemical treatment is required.


**  Stability +  substrates allow treatment in different chemical environments.

Stability –  substrates do not allow treatment in different chemical environments. Only deposition and drying of water, alcohol or chloroform solutions are possible.

***  Recommended power density  depends on substance.

SERS Analysis

Quantitative and qualitative SERS-analysisColloidSERS11

Surface-enhanced Raman spectroscopy is an ultra-sensitive method of identification of pure chemical compounds whereas clinical samples (blood, serum, fecal extracts, cerebrospinal fluid, saliva, urine, etc.) are complex mixtures of organic and inorganic compounds, biomolecules and microorganisms. Direct detection of markers of infectious diseases by SERS analysis is not possible without application of special approaches to eliminate the background (nonspecific) signal. The problem is solved using bioaffinity interactions and SERS reporters as well as label-free techniques.

EnSpectr SERS (Surface Enhanced Raman Scattering) Substrates enable identification of various chemical compounds in extremely low quantities (up to several molecules) based on the effect of giant Raman resonance. EnSpectr SERS Substrates can be used in everyday chemical analysis as well as in fundamental scientific research.  Here are some examples of SERS applications:ColloidSERS2.1

  • Research of cells, tissues, DNA
  • Bioscience and pharmaceutical research
  • Product authentication with spectral markers technology, etc.
  • Detection of prohibited substances


The EnSpectr SERS® is a unique instrument that combines the advantage of a portable probe system with the performance of a highly specified laboratory instrument. It is a perfect choice for SERS analysis when acquisition of high quality data averaged throughout a large area of an active SERS substrate zone is essential.

For the comparative analysis you place the SERS substrate to the sample stage, than you’ll need to do the normalization measurement on one SERS zone of substrate -place the calibration substance on one SERS zone and, having fixed the substrate on the stage, push into the tip  (position 2). And then make the measurement of the test substance on the second SERS zone (which is identical to the first SERS zone) of coupled substrate  – push the stage to make measurement (position3) .




EnSpectr SERS Raman Analyzer Specification
Laser Wavelength 532 nm
Laser Power 0.3-30 mW
Spectral Range 100 cm-1 – 4000 cm-1
Spectral Resolution 7-12 cm-1
Detector Type Linear CCD Array
Pixel Number 3648
Pixel Size 8 μm x 200 μm
Dark Current 630 e/pixel/s
Readout Noise 30 e rms
Dynamic Range 2100
Max Quantum Efficiency 90%
Integration Time 10 ms – 500000 ms
Optical Bench
Focal Length 75 mm
Entrance Aperture 50 (30 -optional) μm wide slit
Grating 1800 g/mm holographic grating
USB 1 External Port 2.0
Power Input 100 – 240 VAC, 50 –60 Hz
System Requirements Windows XP/Vista/7/8
Dimensions 222 mm x 145 mm x 55 mm
Weight 1.5 kg